|
二维码（扫一下试试看！） |
| 高效液相色谱法指纹图谱结合化学模式识别对除湿丸质量控制研究 |
| Study on quality control of Chushi Wan by high performance liquid chromatography fingerprint combined with chemical pattern recognition |
| 收稿日期：2023-05-18 |
| DOI：10.20047/j.issn1673-7210.2024.03.02 |
| 关键词: 除湿丸 指纹图谱 聚类分析 主成分分析 |
| Key Words: |
| 基金项目:国家自然科学基金资助项目（81774309）；北京市中医药研究所市财政专项资助课题（YJS-2022-20） |
|
| 摘要点击次数: 85 |
| 全文下载次数: 80 |
| 摘要:目的 建立除湿丸指纹图谱，为完善其质量控制提供参考。 方法 采用高效液相色谱法（HPLC），色谱柱为Luna Omega Polar C18色谱柱（150 mm×4.6 mm，5 μm），流动相：乙腈-0.2%磷酸水溶液，流速为1.0 ml/min，波长为254 nm，柱温30 ℃，进样量10 μl。以黄芩素为参照，测定29批除湿丸的HPLC图谱，并采用中药色谱指纹图谱相似度评价系统（2012版）进行相似度评价。 结果 29批除湿丸HPLC指纹图谱有35个共有峰，相似度均＞0.95，并指认了8个色谱峰；聚类分析将29批除湿丸聚为3类；主成分分析中7个主要成分分别反映32个共有峰信息。 结论 不同批次除湿丸存在一定的质量差异。所建立的除湿丸指纹图谱可全面评价除湿丸的质量。 |
| Abstract:Objective To establish the fingerprint of Chushi Wan and to provide the reference for improving the quality control. Methods Luna Omega Polar C18 column（150 mm ×4.6 mm，5 μm） was used in this high performance liquid chromatography(HPLC), mobile phase: acetonitrile-0.2% phosphoric acid aqueous solution, flow rate was 1.0 ml/min, wavelength was 254 nm, column temperature was 30 ℃, injection volume was 10 μl. The HPLC chromatograms of 29 batches of Chushi Wan were determinated and evaluated by the similarity evaluation system of traditional Chinese medicine chromatographic fingerprint (2012 edition) with the reference of baicalein. Results There were 35 common peaks in the HPLC chromatograms of 29 batches of samples, the similarity was above 0.95 and eight chromatographic peaks were identified. Cluster analysis grouped 29 batches of Chushi Wan into three groups. The seven main components in principal component analysis reflected 32 common peaks information. Conclusion There are some quality differences in different batches of Chushi Wan. The established fingerprint can be used to evaluate the quality of Chushi Wan. |
| 查看全文 HTML 查看/发表评论 下载PDF阅读器 |
