| 郭喜平,王旭昀,卢冬冬,俎亚杰,张耀圣.高脂血症大鼠前列腺增生模型的建立[J].中国医药导报,2024,21(4):16-20 |
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| 高脂血症大鼠前列腺增生模型的建立 |
| Establishment of prostatic hyperplasia model in hyperlipidemia rats |
| 收稿日期： 修订日期：2023-09-21 |
| DOI：10.20047/j.issn1673-7210.2024.04.04 |
| 关键词: 高脂血症 大鼠 前列腺增生 炎症反应 病理变化 |
| Key Words: |
| 基金项目:北京市优秀人才培养资助青年骨干个人项目（2016000082595G278） |
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| 摘要:目的 构建高脂血症大鼠前列腺增生模型，探讨高脂血症大鼠前列腺增生病理状态。 方法 3月龄SPF级SD雄性大鼠20只（体重 180～200 g）采用随机数字表法分为对照组10只，实验组10只。对照组10只大鼠普通饲料喂养10周；实验组10只大鼠高脂饲料饲养10周，构建高脂血症大鼠前列腺增生模型。氧化酶偶联比色终点法检测血清总胆固醇（TC）、甘油三酯（TG）；消除法检测高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）；ELISA法检测前列腺特异性抗原（PSA）、血清瘦素（LEP）、白细胞介素（IL）-6、IL-8；苏木精-伊红染色法检测前列腺组织变化。结果 实验组TC、TG、LDL-C高于对照组，差异有统计学意义（P＜0.05）；实验组HDL-C低于对照组，差异有统计学意义（P＜0.05）。实验组体重低于对照组，差异有统计学意义（P＜0.05）；实验组前列腺湿重、前列腺指数高于对照组，差异有统计学意义（P＜0.05）。实验组LEP、IL-6、IL-8、PSA高于对照组，差异有统计学意义（P＜0.05）。结论 高脂饲料大鼠前列腺组织增生，炎症反应是其重要病理变化。 |
| Abstract:Objective To establish hyperlipidemia rats prostatic hyperplasia model and to explore the pathological state of hyperlipidemia rats prostatic hyperplasia. Methods A total of 20 SPF SD male rats aged three months (body weight 180-200 g) were divided into the the control group (10) cases and experimental group (10) cases by random number table method. A total of 10 rats in control group were fed ordinary diet for 10 weeks; 10 rats in the experimental group were fed high-fat diet for 10 weeks, and the hyperlipidemia rat prostatic hyperplasia model was established. Serum total cholesterol (TG) and triglyceride (TG) were detected by oxidase-coupled colorimetric end point method. High density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) were detected by elimination method. Prostate-specific antigen (PSA), serum leptin (LEP), interleukin(IL)-6, and IL-8 were detected by ELISA. The changes of prostate tissue were detected by hematoxylineosin staining. Results TC, TG, and LDL-C in experimental group were higher than those in control group, and the differences were statistically significant (P＜0.05). HDL-C in the experiment group was lower than that in the control group, and the difference was statistically significant (P＜0.05). The weight of the experimental group was lower than that of the control group, and the difference was statistically significant (P＜0.05). The wet weight and prostatic index of the experimental group were higher than those of the control group, and the differences were statistically significant (P＜0.05). LEP, IL-6, IL-8, and PSA in the experimental group were higher than those in the control group, and the differences were statistically significant (P＜0.05). Conclusion Hyperplasia of prostate tissue in rats with high fat diet and inflammation are the important pathological changes. |
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